International Journal of Medicine Research

International Journal of Medicine Research


International Journal of Medicine Research
International Journal of Medicine Research
Vol. 1, Issue 2 (2016)

Tuberculosis diagnosis by staining, isolation and PCR from bovine milk samples


Shivaraj S Aralikatti, Rathnamma D, Renuka Prasad C, Shri Krishna Isloor, Chandranaik BM, Narayan Bhat. M.

Tuberculosis is a age old disease in humans and animals. Being zoonotic disease plays a role establishing a considerable impact in maintaining the health of nation in terms of healthy human resource and profitable (healthy) animal resource. A complete eradication needs a comprehensive approach from both human and animal sector. Milk is a major source for large scale spread of tuberculosis infection to humans next only to spread among infected and healthy individuals. Thanks to Louis Pasteur and Pasteurization on advent of which the tuberculosis cases and reports from milk might have reduced considerably. But still due to some myths about raw milk consumption posing the susceptible population to milk borne infections made the work and article to focus on the aspect. In India after eradicating polio, central health ministry is concentrating on TB eradication by advertising on public platforms regarding the disease and the need of eradication endorsed by Mr. Amithab Bachan who once suffered the disease, recovered after proper diagnosis and scheduled treatment. Age old Gold standard staining and isolation techniques along with PCR was performed on milk samples with BCG as positive control for organisms belonging to Mycobacterium Tuberculosis Complex (MTC) group. Effective quarantine measures, diagnosis of disease, concept of clean milk production with wiping of udder with clean cloth before applying milking machines in a dairy herd might have enabled the control of tuberculosis infection in the selected farm.
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How to cite this article:
Shivaraj S Aralikatti, Rathnamma D, Renuka Prasad C, Shri Krishna Isloor, Chandranaik BM, Narayan Bhat. M.. Tuberculosis diagnosis by staining, isolation and PCR from bovine milk samples. International Journal of Medicine Research, Volume 1, Issue 2, 2016, Pages 133-135
International Journal of Medicine Research