The methanolic extract of leaves of aster lanceolatus was assessed for its phytochemical screening, in vitro anti-microbial and anti-inflammatory activity. Phytochemical analysis of aster lanceolatus plant extracts revealed the presence of various biochemical compounds such as alkaloids, flavonoids, glycosides, triterpenoids and saponins etc. Since triterpenoids and flavonoids have remarkable anti-inflammatory activity. The methanolic leaf extract of aster lanceolatus gave good antimicrobial and anti-inflammatory activity. In vitro anti-inflammatory activity was evaluated using albumin denaturation assay, proteinase inhibitory activity, membrane stabilization, heat induced and hypotonicity induced haemolysis at different concentrations. Diclofenac sodium was used as standard drugs. The results obtained in the present study indicate that methanol extracts of aster lanceolatus can be a potential source of anti-inflammatory agents.