Vol. 4, Issue 4 (2019)
Study of thrombolytic and neuropharmacological activities of crude extract of Achyranthes asperaleaves and Piper nigrum fruits
Author(s): Mohidul Islam, Mahmudul Hasan Itu, Mohammad Jamir Uddin, Md. Kaiser, Mehedi Hasan, Koushik Barua, Md. Safayet Hosssain, Dipta Barua Talukder, Fowzul Islam Fahad, Md. Ahsan Ullah, Mahfuz Ahmed Sakib, Md. Ashraf Uddin Chowdhury
Abstract: This study aimed to evaluate the in vitro thrombolytic potential and in-vivo neuropharmacological activities of methanol extract of Achyranthes asperaleaves (MEAAL) and Piper nigrum fruits (MEPNF). The in-vitro thrombolytic activity was determined by the clot lysis method, and in vivo, neuropharmacological activity (antidepressant) was determined by the tail suspension test. The in-vitro thrombolytic activity study revealed that methanol extract of Achyranthes aspera leaves (MEAAL)Piper nigrum fruits (MEPNF), and combination of these two plant extract showed 29.73±1.21%, 35.4±0.39%, and 24.58±1.37% clot lysis respectively. In tail suspension test the immobile times for methanol extract of Achyranthes aspera leaves were observed to be 114.33±4.66 seconds and 97.66±6.06 seconds for dose 200 mg/kg and 400 mg/kg respectively when compared with control at 204.33±1.76seconds of immobile time. And the immobile times for methanol extract of Piper nigrum fruits were observed to be 118.66±6.11 seconds and 90.66±6.33seconds for dose 200 mg/kg and 400 mg/kg, respectively when compared with control at 204.33±1.76 seconds of immobile time. The outcome demonstrates that methanol extract of Achyranthes asperaleaves and Piper nigrum fruits have moderate thrombolytic activity, it also shows good antidepressant activity.