Cellular pattern analysis and there cyto histopathological correlation by two technique in serous body fluids
Nidhi Verma, Veer Karuna, Priya Gupta
Background: Rendering astir theserous body fluids is slenderly punk by cytology. Now, the cell block (CB) technique is the best method to bonk the cell architecture, easy diagnosis, staging and prognosis. Aim: This study was conducted with the intent of cell pattern approach to construe serous body fluids cytology and to demonstrate diagnostic accuracy of cell block technique with an accent on diagnostic pitfalls. Material and Method: Total 192 serous fluids were evaluated, from the year 2017 to 2018. Two techniques were executed in each fluid severally, conventional cytology and cell block. Cytology slides were made after centrifugation or cytospin technique. For cell block preparation, 95% ethyl alcohol was used. Various predominant cell patterns were noted in each case. Overall the final diagnosis was arrived by observing cellularity, architecture, morphology, nuclear and cytoplasmic details and correlated with histopathology in all cases. The sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and diagnostic accuracy were computed. Result: In conventional cytology, Benign, suspicious for malignancy (SM) and malignant cases were seen in 77.08, 10.94 and 11.98% respectively. Cell block method reveled benign in 76.56% cases and 23.44% in malignant cases. Cytological diagnosis was concordant with histopathological (CB) diagnosis in 167 (86.98%) cases. By cell block malignancy was 11.46% more than CC. Overall sensitivity and specificity were 93.33 and 98.63%, respectively. PPV, NPV and diagnostic accuracy were 95.45, 97.99 and 97.39% respectively. Conclusion: Cell block method helps to make correct and definite diagnosis and raise the sensitivity. We can narrow or reduced unnecessary or invasive surgeries.