Vol. 4, Issue 1 (2019)
Comparison of ALP and LDH levels and role in depicting prognosis in metastatic colorectal carcinomas
Author(s): Dr. Sajad Ahmad Bhat, Dr. Tabinda Ayub Shah, Dr. Mir Nadeem, Dr. Basit Rafeeq, Dr. Gumarova Zhannat
Abstract: Introduction: Cancers are characterised by unregulated cell growth, tissue invasion and metastasis. Colorectal cancer is the third most commonly diagnosed malignancy in men (663,000 cases, 10%) and second in women (5710, 000 cases, 9.4%) worldwide as per data available till 2014 by ICMR. Lactate dehydrogenase (LDH) an enzyme that catalyses the conversion of lactate to pyruvate. LDH is released as cells die and finds its way into the blood. Nearly every type of cancer, as well as many other diseases, can cause LDH levels to be elevated. In metastatic colorectal cancer serum alkaline phosphatase (ALP) levels are frequently elevated. Elevation of ALP levels more than three times of normal range is considered significant for evaluation of colorectal hepatic metastases. Material and Methods: The present study was conducted on 100 subjects of colorectal cancer patients in the department of Biochemistry, Faculty of Medical Science SGT University Budhera Gurugram Haryana. Full history and complete clinical examinations, patients with biopsy of colorectal cancer tissues for histopathological examinations to confirm the diagnosis. Venous 5 ml blood sample was collected using aseptic techniques. Serum was separated from the blood by centrifugation at 3000 rpm for 10 mints. Serum was stored at -80 0C until analysis. The repeated thawing and freezing of serum was avoided. Serum samples were analyzed for LDH and ALP levels. All parameters were quantitatively estimated in serum. Result: The mean age group of colorectal cancer patients was 52.9 ± 10.54 and control was 48.7 ± 11.04. As compared to healthy controls, colorectal cancer patients showed significant increase in mean LDH level (173.66 ± 54.55 vs 189.83 ± 22.9, p< 0.007). ALP level showed highly significant differences in mean of colorectal cancer patients and healthy controls (118.28 ± 21.68 vs 72.08 ± 12.65, p<0.000) Conclusion: The combined use of ALP and LDH may be support to predict the prognosis and metastasis of colorectal cancer patients.