Vol. 4, Issue 1 (2019)
Investigation of changes in the enzymatic function of the liver of rabbits, caused by established exogenous and endogenous toxicosis
Author(s): Vagif Shadlinski, Galib Garayev, Hijran Khidirova, Nariman Movsumov
Abstract: Objective:Identify changes in the enzymatic function of the liver by creating a model of exogenous and endogenous intoxication in the experiment. Materials and methods: The experiments were conducted on 48 rabbits of the genus "Chinchilla". Rabbits were divided into three groups: rabbits, included in group I, were subjected to intoxication by inhalation of HCl vapor. In rabbits included in the 2nd group, a model of intestinal obstruction was created. Speaking about the experimental animals included in group III, it should be noted that they also created a model of exotoxicosis and the solution of riditox was introduced into the abdominal cavity. Rabbits for the control group were intact, and the results obtained from them were considered normal. The activity of the enzymes aspartate aminotransferase (AsAT), alanine aminotransferase (AlAT) and lactate dehydrogenase (LDH) was determined in the blood of rabbits using a “Bioscreen MS 2000” microanalyzer. Results: The results showed that intoxication caused by inhalation of HCl impairs the antitoxic function of the liver, and the resulting toxic substances have a negative effect on the synthesis of liver enzymes. This is manifested in the dynamics of the activity of the enzymes AsAT, AlAT and LDH in the blood. Thus, the activity of the AsAT enzyme in the blood during HCl intoxication was on average increased by 2.1-2.5 times or 110.4-149.4%, AlAT activity increased 1.6-2.5 times or 104.2% - 148.1%. However, the activity of the LDH enzyme in the experiment increased by only 8.8-27.8%. Similar results were observed in the dynamics of intestinal obstruction. Conclusion: It was concluded that intoxication of both exogenous and endogenous origin causes a significant impairment of enzyme synthesis in the liver. Thus, activation of all three enzymes (AsAt, Alat, LDH) in the blood was observed when inhaling HCl vapors (exotoxicosis), as well as in case of intestinal obstruction (endotoxicosis). However, the activity of the LDH enzyme increased insignificantly compared to AsAT and AlAT.