Antibiotics one of most commonly prescribed medications in pediatric group, Infants are most susceptible to diseases due to incomplete of immune system. Antimicrobial especially antibiotics has become a routine use in infant illness may that cause many problems. The rising numbers of antibiotics resistance lead us to judicious the use of antibiotics. In this study, we aim to Evaluation of prescribing pattern of antibiotics in infants group guided by WHO guidelines. Descriptive research conducted at Maternity and Children hospital in Mecca, Saudi Arabia. Sampling 253 infants. Including all the infants' inpatients, excluding outpatients, ICU emergency patients patients with AIDS. Data collected from the parents and medical records. In Conclusion, Antibiotics are mostly prescribed drugs. Parenteral route mostly use than oral route, in our present study antibiotic prescription pattern show there are polypharmacy. The average numbers of antibiotic per prescription were markedly higher than which recommended by WHO. Overuse and inappropriate use of antibiotics, excessive parenteral use of antibiotics administration. As the resistance of antibiotics is increasing, so we have to be selected based on culture report and limited use of antibiotics should be done in patients especially infants. Lastly, we recommend further and larger scope prospective study as this is an important issue to emphasize on to ensure rational use of antibiotics and hence fighting against the distressingly increasing antimicrobial resistance. Recruiting clinical pharmacists is very vital in order to observe the clinical use of antibiotics and to deal with related factors. The WHO should supply updates and guidelines on the use of antibiotics in hospitals.