Determination of the intra erythrocyte Glucose-6-phosphate dehydrogenase in human blood by UV-Visible spectroscopy: Development, validation and application
JN Mwelo, JK Mbinze, ML Diyi, FKK Kodondi, B Gulbis
G6PD deficiency is the most common enzymopathy. It would affect more than 400 million people worldwide. It is sometimes called favism due to possible occurrence of hemolysis after ingestion of beans. The clinical manifestations are associated acute hemolytic crises sometimes chronic. These attacks can be triggered by infections, ingestion of oxidant drugs. The diagnosis of this enzymopathy based on the evaluation of the activity of the enzyme implicated. This implies the use of reliable method capable of giving reliable results. In this work a quantitative method of erythrocyte G6PD by UV-Visible spectroscopy was developed and then validated using the strategy of the total error. The method is valid in the range of dosage of 30 μl (1.5 mg / ml) in 50μl (mg / mL). The validated method has been successfully used in Kinshasa (the capital of Democratic Republic of Congo) to assess the activity of G6PD In people with anemia and newborns. The impact of the deficit was 4% in neonates (10/250) and 3.35% for those with anemia (8/239).