The intake of alcoholic beverages has an important social and historical role and the consequences of its use and abuse are still a reality in the contemporary society. It is related to 30% to 50% of serious and fatal traffic accidents in several countries. Recently it has been observed the popularization of the use of alcoholic beverages in association with energy drinks. Due to its stimulating properties, energy drinks have become very popular especially among young people in Brazil and around the world. Due to the high consume of energy drinks associated or not with alcohol, the aim of this study was to evaluate the effects of using alcohol, energy drink and the mixture of these two drinks in the metabolic profile and behavioral parameters of Wistar rats. Male rats were divided in G1 (control group that was fed and watered), G2 (treated with cachaça mixed with water), G3 (treated with energy drink and water 1:1) and G4 (treated with cachaça and energy drink 1:1). After a period of 45 days, the animals performed the Elevated Plus Maze and after that they were euthanized and blood samples were collected to evaluations of total cholesterol, HDL-c, triglycerides, glycaemia, AST, ALT and CPK. Atherogenic Coefficient (AC), Atherogenic Index (AI), Cardiac Risk Ratio 1 (CRR1), and non-HDL-c levels were also analyzed. Our results regarding food consumption, body parameters, glycaemia, HDL-c, AST, ALT, CPK, AC, CCR1 and non-HDL-c revealed no significant differences among the groups. Significant increase were found for AI, triglycerides and total cholesterol levels in the G2, G3 and G4. Results for behavioral test showed that the use of the drinks significantly modify the frequency in the open and closed arm and in the center. We may conclude that the consumption of alcohol, energy drinks and the association of these beverages may increase the risk for cardiovascular diseases once they increase Atherogenic Index and levels of cholesterol and triglycerides.