A practical and reliable of determination of erythrocyte hexokinase and glucose-6-phosphate dehydrogenase
Mwelo Ngobe JA, Diyi Lobo M, Nzingula P, Mavinga B Mbala, JK Mbinze, Kodondi K, Gulbis B
The erythrocyte enzymopathies are among diseases affecting red blood cell enzymes and several of them are known worldwide: Glucose-6-Phosphate Dehydrogenase deficiency, Pyruvate Kinase deficiency, Hexokinase deficiency, etc... The Glucose-6-Phosphate Dehydrogenase (G6PD) deficiency is most common and most widespread erythrocyte enzymopathy worldwide. In the case of enzyme deficiency, the red blood cells will not be able to properly perform their duties. Enzymes are currently assayed in clinical in order to highlight the enzymopathies cases. Nowadays, the evaluation of the enzymatic activity involved to highlight clinically enzymopathies. This evaluation requires the use of reliable methods. We have conducted this work with the objective of establishing a practical and reliable spectrophotometric assays of hexokinase (HK) and G6PD making an automated method used by the chemistry laboratory of the hospital manual Erasmus. Some validation criteria including repeatability, linearity and intermediate precision were evaluated and showed that the methods are reliable. Comparison of the results from analyzed samples using both the developed method and the reference one by the statistical Student-test hasn’t shown a significant difference between the two methods.